density od 450 Search Results


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Dojindo Labs density od 450
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Thermo Fisher multiscan ex photometer
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DYNEX tech optical density (od) reader
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IDEXX eia optical density (od 450)
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
Eia Optical Density (Od 450), supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mutiskan rc plate reader
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
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Tecan Systems optical density measurement
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
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Bio-Rad imark™
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
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Bio-Rad density od
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
Density Od, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies elisa reader
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
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Danaher Inc elisa microplate reader
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
Elisa Microplate Reader, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Hotgen Biotech Co Ltd enzyme-linked immunosorbent assay kit (iga
The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
Enzyme Linked Immunosorbent Assay Kit (Iga, supplied by Beijing Hotgen Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates

Journal: BMC Veterinary Research

Article Title: Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

doi: 10.1186/s12917-018-1400-9

Figure Lengend Snippet: The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates

Article Snippet: The relative amount of PrP Sc remaining was assessed by the EIA optical density (OD 450 ) after dilution of treated brain homogenates to a final [GdnHCl] of 0.25 M and application to the IDEXX plate.

Techniques: Infection, Incubation, Concentration Assay, Comparison

The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure

Journal: BMC Veterinary Research

Article Title: Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

doi: 10.1186/s12917-018-1400-9

Figure Lengend Snippet: The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure

Article Snippet: The relative amount of PrP Sc remaining was assessed by the EIA optical density (OD 450 ) after dilution of treated brain homogenates to a final [GdnHCl] of 0.25 M and application to the IDEXX plate.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay